Title: Synthesis, DNA binding and equilibrium investigation of Model Pd(II)pip complexes with some selected DNA unit-constituents | ||
Author(s): Hannen H. Elgedany, Azza A. Shoukry | ||
Pages: 1-12 | Paper ID:181606-3939-IJBAS-IJENS | Published: December, 2018 |
Abstract: For the purpose of finding additional metal based anticancer medications with lower toxicity and higher affectivity, Pd(Pip)Cl2 complex, where Pip is piprazine, was synthesized and characterized. The stoichiometries and stability constants of the complexes formed between [Pd(Pip)(H2O)2]2+ and some selected bimolecular targets as DNA unit constituents were investigated at 25°C and at constant 0.1 mol.dm-3 ionic strength. The various complex species formed were evaluated utilizing concentration distribution diagrams. Solid ternary complexes involving [Pd(Pip)]2+ with inosine, guanosine and adenine have been synthesized and characterized , and tested for their biological activity against some selected bacteria and fungi. A further investigation of the binding properties of the diaqua complex [Pd(Pip)(H2O)2]2+ with calf thymus DNA (CT-DNA) was investigated by UV–Vis spectroscopy throughout this research. The intrinsic binding constants (Kb) calculated from UV– Vis absorption studies is 4.68x103 mol.dm-3. The calculated (Kb) value was found to be of lower magnitude than that of the classical intercalator EB (ethidium bromide) (Kb = 1.23 (±0.07) x 105 mol.dm-3), suggesting an electrostatic and/or groove binding mode for the interaction with CT-DNA.
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Keywords: --- | ||
Full Text (.pdf) | 892 KB |
Title: Assessment of Heavy Metals Toxicity in Sediments at lake Edku, Egypt | ||
Author(s): Mohamed A. Okbah, Maie I. El-Gammal, Mahmoud S. Ibrahim, Yousef A. A. Waheshi | ||
Pages: 13-23 | Paper ID:182706-3434-IJBAS-IJENS | Published: December, 2018 |
Abstract: The study is carried out to assess the level
contamination of some heavy metals content (Fe, Mn, Zn, Cu, Ni,
Cr, Co and Pb) in the sediment of Lake Edku. To achieve that
five indices for pollution assessment are used; toxic unit values
(TU), Toxic risk index (TRI), Enrichment Factor (EF), Geoaccumulation Index (Igeo) and Pollution Load Index (PLI). The
results of the total metals concentration are decreased in the
order of Fe > Mn >Cr >Co >Zn > Cu >Ni= Pb. The calculated
values of toxic units (∑TU) of Zn, Cu, Ni, Pb and Cr for all the
sampling sites was lower than 4 and ranged from 6.01 to 9.26
with mean value of (8.02 ±1.09) for TRI, indicating a low toxic
risk of the metals. The enrichment factor has the order of EFCo >
EFMn >EFPb > EFCu >EFCr >EFZn >EFNi. According to the Igeo
index classification, Fe, Zn, Ni and Cr may be practically
unpolluted (Igeo ranged from -2.15 to -0.41) for the sediments
study, while the Igeo of Co ranged from moderately to strongly
polluted. According to the values of PLI (ranged from 1.25 to
1.67), the lake Edku was existed in polluted and moderately
polluted
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Keywords: Sediment, Heavy Metals, Lake Edku, Enrichment Factor | ||
Full Text (.pdf) | 794 KB |
Title: The Effect of Using 1.7 W of 940 nm Diode Laser on Radicular Dentin Permeability and Smear Layer Removal | ||
Author(s): Ruaa M. Al-Mafrach, Lutfi G.Awazli, Mohammed A. Al-Maliky, Ruaa M. Al-Mafrachi | ||
Pages: 24-27 | Paper ID:182906-5757-IJBAS-IJENS | Published: December, 2018 |
Abstract: Background: Near-infrared diode lasers can be used
for several applications, which range from disinfection to smear
layer removal in endodontics. This study aimed to evaluate the
effect of using 1.7 W of 940 nm diode laser on root canal dentin
permeability and removal of the smear layer. Method: twentyeight extracted human mandibular premolars teeth were
instrumented up to X4 file of ProTaper next rotary system
(Dentsply Maillefer, Ballagues, Switzerland) irrigated with 1 ml
5.25% NaOcl between files and then randomly divided into 2
groups (n = 14 for each group) control group and diode laser
group. Afterward, the roots were coated with nail varnish to
made them impermeable and then filled with 2%methylene blue
dye, divided horizontally into three segments representing the
apical, middle, and coronal thirds then examined under stereomicroscope. Using analytical software, the root section area and
dye penetration area were measured, and then, the percentage of
clear dye penetration area was calculated. Moreover, scanning
electron microscope investigations were accomplished. The nonparametric Mann-Whitney U test was accomplished. Although
the statistical analysis showed no significant difference between
the two experimental groups over the three root thirds but the
dye penetration in diode laser group was significantly higher
over the whole root length compared to control group. Scanning
electron micrographs of diode laser group showed a distinctive
removal of smear layer with preservation of the annular
structure of dentinal tubules, while control group produced
uneven removal of smear layer, in efficient cleanliness especially
in the apical third.
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Keywords: Diode laser, Smear layer, Permeability, Root canal. | ||
Full Text (.pdf) | 535 KB |